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Related Experiment Videos

Basic fibroblast growth factor expression in human bone marrow and peripheral blood cells

G Brunner1, H Nguyen, J Gabrilove

  • 1Department of Cell Biology, New York University Medical Center, NY 10016.

Blood
|February 1, 1993
PubMed
Summary

Basic fibroblast growth factor (bFGF) is found in human bone marrow megakaryocytes and platelets, and is deposited in the extracellular matrix. Stromal cells produce bFGF, supporting its role in myelopoiesis and platelet function.

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Area of Science:

  • Hematology
  • Cell Biology
  • Biochemistry

Background:

  • Basic fibroblast growth factor (bFGF) is a known mitogen for human bone marrow (BM) stromal cells.
  • bFGF has been shown to stimulate myelopoiesis in primary BM cultures.

Purpose of the Study:

  • To investigate the presence and localization of bFGF in human BM and peripheral blood cells.
  • To determine the role of BM stromal cells and their extracellular matrix in bFGF storage and release.
  • To explore the potential involvement of bFGF in platelet function and megakaryocytopoiesis.

Main Methods:

  • Immunofluorescence microscopy on BM and peripheral blood smears.
  • Enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation followed by immunoblotting to confirm bFGF presence in platelets.

Related Experiment Videos

  • Analysis of bFGF synthesis, localization (cell-associated, nuclear, nucleoli), and extracellular matrix deposition in BM stromal cell cultures.
  • Investigation of bFGF release from extracellular matrix using heparin and phosphatidylinositol-specific phospholipase C.

    • Main Results:

    • bFGF was strongly detected in megakaryocytes and platelets, with weaker staining in granulocytic cells.
    • BM stromal cells synthesize bFGF, which is found cell-associated, in the nucleus/nucleoli, and deposited in the extracellular matrix.
    • Extracellular binding molecules for bFGF were identified on stromal cells, and bFGF could be released by heparin or phospholipase C.
    • The study supports bFGF's role as a stromal cell mitogen and myelopoiesis stimulator.

    Conclusions:

    • BM stromal cells produce bFGF, and their extracellular matrix acts as a reservoir for this growth factor.
    • bFGF is present in platelets, suggesting a potential role in platelet function and megakaryocytopoiesis.
    • The findings reinforce the significance of bFGF in hematopoiesis and BM microenvironment regulation.