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Related Experiment Videos

A novel short consensus repeat-containing molecule is related to human complement factor H

C Skerka1, S Kühn, K Günther

  • 1Bernhard Nocht Institute for Tropical Medicine, Hamburg, Federal Republic of Germany.

The Journal of Biological Chemistry
|February 5, 1993
PubMed
Summary
This summary is machine-generated.

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Researchers discovered a new human factor H-related gene, DOWN16, in liver cells. This gene encodes a protein with structural similarities to factor H, suggesting potential shared functions in the human serum.

Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • Factor H is a key regulator of the complement system.
  • Factor H-related proteins share structural and functional similarities with factor H.
  • Understanding novel factor H-related proteins is crucial for elucidating complement system regulation.

Purpose of the Study:

  • To identify and characterize a novel factor H-related cDNA from a human liver cDNA library.
  • To analyze the structural features and potential function of the identified gene product.
  • To investigate the relationship between the novel protein and known factor H-related proteins.

Main Methods:

  • Isolation and sequencing of the DOWN16 cDNA clone from a human liver cDNA library.
  • Analysis of the predicted amino acid sequence, including signal sequence and short consensus repeats (SCRs).

Related Experiment Videos

  • In vitro translation to determine the molecular mass and post-translational modifications of the encoded protein.

    • Main Results:

    • Identification of the DOWN16 clone, a 1269 bp cDNA hybridizing to a 1.4 kb mRNA.
    • The predicted 331 amino acid translation product contains a signal sequence and five SCRs homologous to factor H.
    • In vitro translation yielded a 37,500 Da glycosylated protein directed to the secretory pathway, suggesting presence in human serum.

    Conclusions:

    • The DOWN16 gene represents a novel factor H-related protein.
    • Structural homology to factor H suggests potential shared biological functions.
    • Further research is warranted to determine the precise functions of this protein in the human serum and complement system.