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Gas chromatographic determination of trace nitrite after derivatization with ethyl 3-oxobutanoate
1Hyogo Prefectural Institute of Public Health, Arata-cho, Hyogo-Ku, Kobe, Japan.
Journal of Chromatography
|January 22, 1993
Abstract:
A gas chromatographic method was developed for the determination of nitrite using ethyl 3-oxobutanoate as a derivatization reagent. Nitrite was reacted with ethyl 3-oxobutanoate in the presence of hydrochloric acid to form ethyl 2-hydroxyimino-3-oxobutanoate (EHOB) quantitatively. The resulting EHOB was extracted with ethyl acetate and then determined sensitively by gas chromatography with electron-capture detection. This method has been applied successfully to the determination of nitrite in river water and human saliva, with a detection limit of 2 ng/ml and recoveries of 94-100%.