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Large scale recovery of biologically active IgM (95% pure) from human plasma obtained by therapeutic plasmapheresis
1Department of Pathology, SUNY Health Science Center, Syracuse 13210.
We have developed a large scale (10 g/batch) non-chromatographic method for the collection of IgM directly from plasma obtained from therapeutic plasmapheresis of patients. The technique uses sequential precipitations from ammonium sulfate and polyethylene glycol, and gives high yields of undenatured IgM antibodies. The biological activity of IgM prepared by this method has been demonstrated by systemically transfusing animals with the antibody and producing a demyelinating syndrome very similar to the antibody donor. Control IgM preparations exhibited no toxicity after daily injections of antibody for 8 weeks, showing that the method gives antibody with excellent bio-compatibility (Tatum, 1989). The method, which is effective for monoclonal and polyclonal antibodies, is useful when large amounts of antibodies are needed for passive transfer or other studies.
We have developed a large scale (10 g/batch) non-chromatographic method for the collection of IgM directly from plasma obtained from therapeutic plasmapheresis of patients. The technique uses sequential precipitations from ammonium sulfate and polyethylene glycol, and gives high yields of undenatured IgM antibodies. The biological activity of IgM prepared by this method has been demonstrated by systemically transfusing animals with the antibody and producing a demyelinating syndrome very similar to the antibody donor. Control IgM preparations exhibited no toxicity after daily injections of antibody for 8 weeks, showing that the method gives antibody with excellent bio-compatibility (Tatum, 1989). The method, which is effective for monoclonal and polyclonal antibodies, is useful when large amounts of antibodies are needed for passive transfer or other studies.