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Cloning and sequence analysis of the phosphoprotein gene of rinderpest virus
M D Baron1, M S Shaila, T Barrett
1AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
Abstract:
We have cloned several cDNAs derived from the P gene of rinderpest virus. One of these, derived from a bicistronic N-P mRNA, has been sequenced in its entirety. Sequencing of a section of the others, and comparison with the genome sequence, showed that P gene transcripts, as for other morbilliviruses, were variable; non-templated Gs could be added at a site resembling the normal stop transcription site. Primer extension analysis showed that about half the transcripts were edited. Sequences of the P, C and V proteins encoded by the normal and edited transcripts were compared with those of other morbilliviruses and with those of the more distantly related paramyxoviruses.
Insights
Researchers studied rinderpest virus P gene transcripts, finding significant variability and editing. This research enhances understanding of morbillivirus gene expression and protein diversity.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Rinderpest virus is a significant pathogen within the Morbillivirus genus.
- Understanding viral gene expression is crucial for developing effective control strategies.
Purpose of the Study:
- To investigate the molecular characteristics of rinderpest virus P gene transcripts.
- To analyze the variability and editing of these transcripts and their encoded proteins.
Main Methods:
- Cloning and sequencing of complementary DNAs (cDNAs) derived from the P gene.
- Comparative sequence analysis with the rinderpest virus genome.
- Primer extension analysis to detect transcript editing.
- Sequence comparison of encoded proteins (P, C, V) with other morbilliviruses and paramyxoviruses.
Main Results:
- Several P gene cDNAs were cloned, with one bicistronic N-P mRNA fully sequenced.
- P gene transcripts exhibited variability, including the addition of non-templated guanosines (Gs) at transcription termination sites.
- Approximately half of the transcripts analyzed showed evidence of editing.
- Comparative analysis revealed similarities and differences in P, C, and V proteins among morbilliviruses and paramyxoviruses.
Conclusions:
- Rinderpest virus P gene transcripts display significant post-transcriptional modification, including variability and editing.
- These modifications contribute to the diversity of P, C, and V proteins, impacting viral biology.
- The findings provide insights into the molecular mechanisms of morbillivirus gene expression.