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Related Experiment Videos

Cellular responses to silicone and polyurethane prosthetic surfaces

A Sank1, J Chalabian-Baliozian, D Ertl

  • 1Department of Surgery, LAC+USC Medical Center 90033.

The Journal of Surgical Research
|January 1, 1993
PubMed
Summary

Prosthetic materials like silicone and polyurethane reduce cell attachment and proliferation, contributing to fibrous capsule formation around surgical implants. This study investigated cellular responses to these common medical device surfaces.

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Prosthetic devices made of silicone or polyurethane are widely used in surgery.
  • Capsule formation is a common complication, involving fibroblasts and endothelial cells (EC) interacting with collagen type I.
  • The study investigates how prosthetic material surfaces alter cellular function, potentially leading to capsule formation.

Purpose of the Study:

  • To determine the impact of prosthetic material surfaces on endothelial cell (EC) and fibroblast function.
  • To investigate the role of altered cellular function in the development of fibrous capsules around implants.
  • To compare the cellular responses to foam polyurethane, flat silicone, and textured silicone surfaces.

Main Methods:

  • In vitro assays were used to measure cell attachment and proliferation.

Related Experiment Videos

  • Scanning electron microscopy (SEM) evaluated morphologic changes on prosthetic surfaces.
  • Human umbilical vein endothelial cells (HUVEC) and fibroblasts were exposed to different prosthetic materials.

    • Main Results:

    • Cell attachment for both EC and fibroblasts was significantly reduced on all tested prosthetic surfaces compared to controls.
    • Fibroblast proliferation decreased on foam polyurethane and textured silicone but not flat silicone.
    • EC proliferation was significantly reduced on both silicone surfaces but not on polyurethane.

    Conclusions:

    • Prosthetic material surfaces significantly impair EC and fibroblast attachment and proliferation.
    • Altered cellular function in response to biomaterials is a key factor in capsule formation.
    • The specific material properties (e.g., texture, composition) influence the degree of cellular response.