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Related Experiment Videos

Complement activation in stored platelet concentrates

V D Miletic1, O Popovic

  • 1Department of Tissue Typing and Immunochemistry, Blood Transfusion Institute, Belgrade, Yugoslavia.

Transfusion
|February 1, 1993
PubMed
Summary
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Platelet activation during storage may be caused by complement activation. Complement activation products were detected in stored platelet concentrates, indicating a potential mechanism for platelet activation.

Area of Science:

  • Immunology
  • Hematology
  • Biochemistry

Background:

  • Platelet concentrates stored at room temperature can activate platelets.
  • The complement system is a potential biologic cause of this platelet activation.

Purpose of the Study:

  • To investigate complement activation in platelet concentrates during storage.
  • To detect complement activation products and functional complement activity.

Main Methods:

  • Functional hemolytic assays for classical and alternative complement pathways.
  • Kinetic assay for complement-mediated inhibition of immune complex precipitation.
  • Detection of C3 activation products (C3, C3c, C3dg) in plasma and on platelets using SDS-PAGE, Western blotting, and immunofluorescence.

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Main Results:

  • Complement activation was evident during 5 days of room temperature storage.
  • C3c and C3d fragments were detected in platelet concentrate plasma.
  • C3d fragments accumulated on platelets during the first 3 days, then disappeared by day 5.

Conclusions:

  • Complement activation occurs in stored platelet concentrates.
  • Complement activation generates ligands that may mediate platelet activation.
  • Complement activation is a potential mechanism for platelet activation during storage.