Paracetamol estimation: comparison of a quick colorimetric method with a standard spectrophotometric method

Area of Science:

• Clinical Chemistry
• Analytical Chemistry
• Pharmacology

Background:

• Plasma paracetamol (acetaminophen) levels are crucial for therapeutic monitoring and overdose management.
• Existing spectrophotometric methods, while standard, can be susceptible to interference from co-administered drugs.

Purpose of the Study:

• To evaluate a modified colorimetric method for plasma paracetamol estimation.
• To compare its performance against a standard spectrophotometric method.
• To assess the suitability of the colorimetric method as a clinical alternative.

Main Methods:

• A modified colorimetric assay for plasma paracetamol was developed.
• Results were compared with those obtained using a standard spectrophotometric technique.
• Assays for precision, accuracy, and drug interference were conducted.

Main Results:

• The colorimetric method demonstrated good correlation with the spectrophotometric method.
• No significant differences were observed between the two methods.
• The colorimetric assay exhibited acceptable precision, accuracy, and recovery.
• It showed reduced susceptibility to interference from other drugs compared to spectrophotometry.

Conclusions:

• The modified colorimetric method is a reliable and viable alternative for plasma paracetamol quantification.
• Its speed, simplicity, and reduced interference make it advantageous for clinical settings.