Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Amino acid loss during volume regulatory decrease in cultured chick heart cells

R L Rasmusson¹, D G Davis, M Lieberman

¹Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710.

The American Journal of Physiology

|January 1, 1993

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Observation method identifies that a lack of canonical babbling can indicate future speech and language problems.

Acta paediatrica (Oslo, Norway : 1992)·2017

Same author

Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America.

Plant cell reports·2013

Same author

The relationship of nitrogen source and in vivo nitrate reductase actiity to root formation in Euphorbia esula cell suspension cultures.

Plant cell reports·2013

Same author

Electroanalysis and coulometric analysis.

Analytical chemistry·2012

Same author

Bisphosphonates induce senescence in normal human oral keratinocytes.

Journal of dental research·2011

Same author

H-2 antigen expression: Loss in vitro, restoration in vivo, and correlation with cell-mediated cytotoxicity in a mouse lymphoma cell line.

Immunogenetics·2011

Same journal

Blood coagulation in fish.

The American journal of physiology·2011

Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010

Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010

Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010

Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010

Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010

See all related articles

Cultured chick heart cells regulate volume by releasing amino acids like taurine and glutamate during hyposmotic stress. This regulatory volume decrease (RVD) is crucial for maintaining cell function.

Area of Science:

Cardiovascular Physiology
Cell Biology
Biochemistry

Background:

Cardiac cells face osmotic challenges that can impair function.
Understanding cellular volume regulation is key to maintaining cardiac health.

Purpose of the Study:

To investigate the mechanisms of volume regulation in cultured chick heart cells under hyposmotic conditions.
To identify the specific solutes released during regulatory volume decrease (RVD).

Main Methods:

Utilized optical, biochemical, and nuclear magnetic resonance (NMR) methods.
Measured inorganic cation loss using atomic absorption spectroscopy.
Quantified organic solute loss via 1H-NMR and high-pressure liquid chromatography (HPLC).

Main Results:

Related Experiment Videos

Cells swelled and initiated RVD within 3 minutes under hyposmotic stress.
RVD involved the substantial loss of amino acids (taurine, glutamate, aspartate, glycine) and inorganic ions (Na+, K+).
Hyposmotic swelling also stimulated glutamate and aspartate production.

Conclusions:

Cardiac cell volume regulation under hyposmotic stress is mediated by the release and production of amino acids.
These processes are vital for preserving the metabolic and functional integrity of cardiac cells.