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A simple method for isolation of DNA from blood clots suited for use in PCR
R Zeillinger1, C Schneeberger, P Speiser
1First Department of Obstetrics & Gynecology, University of Vienna, Austria.
Biotechniques
|February 1, 1993
Abstract:
We describe a rapid, simple and inexpensive method for the isolation of DNA from blood clots suited for use in PCR. Our method is based on the lysing and nuclease-inactivating properties of guanidine thiocyanate together with the nucleic acid-binding properties of silica particles. Isolated DNA can be used for in vitro amplification as shown for a retinoblastoma gene PCR system.