Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Artifactual variation in randomly amplified polymorphic DNA banding patterns

D L Ellsworth1, K D Rittenhouse, R L Honeycutt

  • 1Department of Wildlife & Fisheries Sciences, Texas A&M University, College Station 77843.

Biotechniques
|February 1, 1993
PubMed
Summary

Randomly amplified polymorphic DNA (RAPD) assays use PCR to detect DNA variations, offering potential as genetic markers. However, reaction inconsistencies can create artifactual data, requiring careful discrimination from true genetic polymorphism for reliable population studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advances in Higher Level Mammalian Relationships.

Cladistics : the international journal of the Willi Hennig Society·2021
Same author

Lifestyle modification interventions differing in intensity and dietary stringency improve insulin resistance through changes in lipoprotein profiles.

Obesity science & practice·2016
Same author

Cardiometabolic risk reduction in an intensive cardiovascular health program.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2012
Same author

Polymorphism and gene organization of water buffalo MHC-DQB genes show homology to the BoLA DQB region.

Animal genetics·2011
Same author

Assessing substitution patterns, rates and homoplasy at HVRI of Steller sea lions, Eumetopias jubatus.

Molecular ecology·2009
Same author

Glacial refugia and the phylogeography of Steller's sea lion (Eumatopias jubatus) in the North Pacific.

Journal of evolutionary biology·2006

Area of Science:

  • Molecular Biology
  • Genetics
  • Population Genetics

Background:

  • Randomly amplified polymorphic DNA (RAPD) and arbitrarily primed PCR (AP-PCR) are DNA polymorphism detection methods.
  • These techniques utilize PCR to amplify random DNA segments with arbitrary oligonucleotide primers.
  • The resulting DNA polymorphisms show Mendelian inheritance, indicating their utility as genetic markers.

Purpose of the Study:

  • To introduce and describe Randomly Amplified Polymorphic DNA (RAPD) and Arbitrarily Primed PCR (AP-PCR) as novel DNA polymorphism assays.
  • To highlight the potential of these assays as genetic markers across various scientific fields.
  • To address the challenges and limitations associated with these techniques, particularly concerning data reliability.

Main Methods:

Related Experiment Videos

  • Employing PCR to amplify random DNA segments using oligonucleotide primers of arbitrary sequences.
  • Analyzing the amplification products to identify DNA polymorphisms.
  • Investigating the inheritance patterns of detected polymorphisms.

    • Main Results:

    • RAPD and AP-PCR assays generate DNA fragments that exhibit Mendelian inheritance.
    • These polymorphisms have significant potential for application as genetic markers.
    • Amplification profiles are sensitive to reaction conditions, leading to potential inconsistencies.

    Conclusions:

    • RAPD and AP-PCR are valuable tools for detecting DNA polymorphisms with potential as genetic markers.
    • The reliability of these assays depends on controlling reaction parameters to avoid artifactual variation.
    • Careful discrimination between true polymorphism and artifacts is crucial for accurate genetic studies in natural populations.