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Generation of somatic cell hybrids capable of proliferating and secreting human monoclonal antibody without any

H Hagiwara1, H Yuasa

  • 1Hagiwara Institute of Health, Hyogo, Japan.

Human Antibodies and Hybridomas
|January 1, 1993
PubMed

A novel cell line called Trioma, which can proliferate and secrete a human monoclonal antibody in the basal medium, was established. Trioma was generated by somatic cell hybridization with human x human hybridoma and A431c, which is a cell line able to grow autonomously in the basal medium. A Trioma called TriH8 has been kept growing in the DMEM/F-12 medium for over 1 year and stably producing stomach cancer-reactive human IgM into culture medium at 10 micrograms/ml. TriH8 has a characteristic cytological phenotype, that is, to diminish cell growth in the presence of epidermal growth factor.

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