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Updated: Aug 5, 2026
09:31
Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
Published on: March 22, 2016
Genomic mapping of intracisternal A-particle proviral elements
K K Lueders1, W N Frankel, J A Mietz
1Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Summary
Researchers mapped 44 Intracisternal A-particle (IAP) proviral loci in the mouse genome. These IAP LS loci offer new genetic markers for the mouse genome map, aiding future research.
Area of Science:
- Genomics
- Molecular Biology
- Mouse Genetics
Background:
- Intracisternal A-particle (IAP) proviral elements are moderately reiterated and dispersed throughout the mouse genome.
- Specific IAP element subfamilies (LS elements) are transcriptionally active in mouse B- and T-cells.
Purpose of the Study:
- To derive oligonucleotide probes from LS elements for genetic mapping.
- To establish chromosomal assignments for LS proviral loci in the mouse genome.
Main Methods:
- Utilized LS element-specific oligonucleotide probes to identify restriction fragments at proviral-flanking DNA junctions.
- Analyzed strain distribution patterns (SDPs) of these fragments across different mouse strains (BXD and CXB RI sets).
- Correlated SDPs with known genetic markers to determine chromosomal locations.
Main Results:
- Identified and mapped 44 LS proviral loci to specific chromosomal locations.
- Demonstrated polymorphic SDPs for these loci across various mouse strains.
- Confirmed the utility of LS elements as genetic markers.
Conclusions:
- The mapped IAP LS loci represent a significant addition of genetically organized markers to the mouse genome map.
- These markers can facilitate further genetic studies and genome analysis in mice.
- LS elements provide valuable tools for understanding mouse genome organization and evolution.
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