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Related Experiment Videos

RNA editing of a chimeric maize mitochondrial gene transcript is sequence specific

R Kumar1, C S Levings

  • 1Department of Genetics, North Carolina State University, Raleigh 27695-7614.

Current Genetics
|February 1, 1993
PubMed
Summary

Maize mitochondrial RNA editing in the C male-sterile cytoplasm (cms-C) C-atp6 gene introduces 19 C-to-U alterations. These edits create a translational stop codon, demonstrating sequence-specific RNA editing independent of gene rearrangements.

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Area of Science:

  • Molecular Biology
  • Plant Genetics
  • Mitochondrial Genetics

Background:

  • Mitochondrial gene expression in plants is often modulated by post-transcriptional RNA editing.
  • The C male-sterile cytoplasm (cms-C) of maize contains a chimeric atp6 gene (C-atp6) resulting from a triple gene fusion.
  • Understanding RNA editing in chimeric genes is crucial for deciphering gene function and evolution.

Purpose of the Study:

  • To analyze the patterns and characteristics of RNA editing in the maize C-atp6 mitochondrial transcripts.
  • To investigate whether gene fusion and rearrangements influence RNA editing specificity.
  • To compare RNA editing events in C-atp6 with other plant atp6 genes.

Main Methods:

  • Sequencing of complementary DNAs (cDNAs) derived from maize C-atp6 transcripts.

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  • Comparative analysis of cDNA sequences with the genomic sequence of C-atp6.
  • Comparison of editing patterns with atp6 transcripts from other plant species (sorghum, Oenothera, Petunia, wheat).

    • Main Results:

    • Nineteen C-to-U RNA editing events were identified in the C-atp6 transcripts, leading to 16 amino acid changes.
    • The region homologous to atp9 showed one edit, while the region of unknown origin was unedited.
    • The atp6-homologous region exhibited 18 editing events similar to sorghum atp6 transcripts, including the introduction of a translational stop codon at a conserved position.

    Conclusions:

    • RNA editing in maize C-atp6 is sequence-specific and not affected by the chimeric nature or rearrangements of the gene.
    • The conserved placement of the translational stop codon via RNA editing suggests functional importance across species.
    • RNA editing plays a significant role in modulating gene expression and potentially restoring functionality in chimeric mitochondrial genes.