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Human PCK1 encoding phosphoenolpyruvate carboxykinase is located on chromosome 20q13.2
H Yu1, R Thun, S Chandrasekharappa
1Department of Human Genetics, University of Michigan, Ann Arbor 48109.
Genomics
|January 1, 1993
Abstract:
Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP) (PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA and to screen a YAC library of human genomic DNA. The sequences of human and rat PEPCK cDNA differed at 16% of the nucleotides compared (45/291). Analysis of a human/rodent hybrid mapping panel demonstrated concordant segregation of PCK1 with chromosome 20. Fluorescence in situ hybridization with YAC DNA further localized PCK1 to subband 20q13.2.