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Fast DNA isolation from Histoplasma capsulatum: methodology for arbitrary primer polymerase chain reaction-based
J P Woods1, D Kersulyte, W E Goldman
1Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110.
Journal of Clinical Microbiology
|February 1, 1993
Abstract:
The arbitrary primer polymerase chain reaction (also called random amplified polymorphic DNA, or RAPD) is a DNA fingerprinting method that provides an efficient, sensitive way of discriminating between independent isolates of Histoplasma capsulatum, but its widespread application has been hampered by the arduous 2-day procedure traditionally used to extract DNA from H. capsulatum. We present here a quick (approximately 2-h) extraction method and show that the resultant DNA is suitable for sensitive and reproducible identification of individual strains of this pathogenic fungus.