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Related Experiment Videos

Plasma volume estimation using indocyanine green. A single intravenous injection method

M W Busse1, S Zisowsky, S Henschen

  • 1Department of Physiology, Hannover Medical School, Germany.

Anaesthesia
|January 1, 1993
PubMed
Summary

This study validates plasma volume estimation using indocyanine green (ICG), finding it highly reliable for clinical use. Despite minor variations, ICG offers accurate and repeatable plasma volume measurements.

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Area of Science:

  • Cardiovascular physiology
  • Medical diagnostics

Background:

  • Accurate plasma volume measurement is crucial for managing fluid balance in critical care.
  • Existing methods for plasma volume estimation have limitations.

Purpose of the Study:

  • To investigate the validity and reliability of plasma volume estimation using indocyanine green (ICG).
  • To compare ICG-based plasma volume measurements with a reference method.

Main Methods:

  • In vitro experiments to assess accuracy against known volumes.
  • In vivo studies comparing ICG with Cr51-labeled red cells for plasma volume estimation.
  • Repeated measurements to assess reliability and response to fluid challenges.

Main Results:

  • In vitro measurements showed high accuracy with approximately 1% error.

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  • ICG measurements closely correlated with Cr51-labeled red cells (r = 0.97), with a 1.7% difference.
  • High reliability was observed in successive measurements (r = 0.99).
  • Plasma volume increased significantly after hyperosmolar saline bolus, consistent with both ICG and plasma protein changes.

    • Conclusions:

    • Indocyanine green is a valid and reliable method for plasma volume estimation.
    • The method demonstrates high accuracy and repeatability.
    • Clinical application is primarily suited for intra-operative or emergency settings due to technical requirements.