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Related Experiment Videos

Lactate transport in macrophages

J D Loike1, E Kaback, S C Silverstein

  • 1Rover Physiology Laboratories, Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032.

Journal of Immunology (Baltimore, Md. : 1950)
|March 1, 1993
PubMed
Summary

Macrophages can absorb L-lactate, especially in acidic environments, using a specific transporter. This lactate serves as an important energy source for macrophage function.

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Area of Science:

  • Immunology
  • Cellular Physiology
  • Biochemistry

Background:

  • Macrophages are key immune cells involved in phagocytosis and effector functions.
  • Inflammatory environments are often acidic and rich in lactate.
  • Understanding macrophage energy metabolism is crucial for immune response.

Purpose of the Study:

  • To investigate the ability of macrophages to accumulate and utilize lactate.
  • To characterize the mechanism of lactate uptake in macrophages.
  • To determine if lactate can serve as an energy source for macrophages.

Main Methods:

  • Utilized thioglycollate-elicited mouse peritoneal macrophages.
  • Measured L-lactate uptake using radiolabeled L-[14C]Lactate.
  • Assessed intracellular phosphocreatine levels under different metabolic conditions (glucose, lactate, no glucose).

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  • Investigated the effect of pH, probenecid, and extracellular Na+ on lactate uptake.

    • Main Results:

    • Macrophage lactate uptake is stereospecific for L-lactate.
    • Uptake is enhanced in acidic extracellular conditions (pH 6.0-6.5) and mediated by a proton cotransport system.
    • Lactate uptake is inhibited by probenecid, indicating a monocarboxylate transporter mechanism.
    • Macrophages can maintain intracellular phosphocreatine stores using lactate when glucose is absent, though less efficiently than with glucose.

    Conclusions:

    • Macrophages actively transport L-lactate from the extracellular environment in a pH-dependent manner.
    • Lactate uptake is mediated by a probenecid-sensitive monocarboxylate transporter.
    • Lactate can be utilized by macrophages as an alternative energy source, particularly in acidic inflammatory settings.