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Related Experiment Videos

nir1, a conditional-lethal mutation in barley causing a defect in nitrite reduction

E Duncanson1, A F Gilkes, D W Kirk

  • 1Division of Environmental and Evolutionary Biology, School of Biological and Medical Sciences, University of St. Andrews, Scotland, UK.

Molecular & General Genetics : MGG
|January 1, 1993
PubMed
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A mutation in barley

Area of Science:

  • Plant molecular genetics
  • Nitrogen metabolism in plants
  • Enzyme function and regulation

Background:

  • Nitrate assimilation is a crucial process in plant growth.
  • Nitrite reductase (NiR) is a key enzyme in this pathway.
  • Understanding the genetic control of NiR is vital for crop improvement.

Purpose of the Study:

  • To identify and characterize genes involved in nitrite reduction in barley.
  • To investigate the genetic basis of nitrite accumulation in leaves.
  • To elucidate the role of the Nir1 gene in barley nitrogen metabolism.

Main Methods:

  • Mutagenesis of barley (Hordeum vulgare L.) with azide.
  • Screening of M2 plants for leaf nitrite accumulation.
  • Genetic analysis of mutant lines, including F2 progeny analysis.

Related Experiment Videos

  • Biochemical assays for nitrite reductase and nitrate reductase activity.
  • Immunological detection of nitrite reductase cross-reacting material.

    • Main Results:

    • Eleven green mutants accumulating nitrite were identified.
    • A single recessive nuclear gene, Nir1, was responsible for nitrite accumulation.
    • Homozygous nir1 mutants showed reduced nitrite reductase activity and apoprotein levels.
    • NADH-nitrate reductase activity was elevated in nir1 mutants.
    • Nir1 mutants were viable in liquid culture but died in compost.

    Conclusions:

    • The Nir1 locus controls the formation of nitrite reductase apoprotein in barley.
    • Nir1 likely represents the structural gene for nitrite reductase or a regulatory gene essential for its synthesis.
    • This finding provides insights into the genetic regulation of nitrogen assimilation in plants.