Experimental application of top-down control analysis to metabolic systems
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Updated: Nov 7, 2026

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains
Published on: August 7, 2018
1Department of Biochemistry, University of Cambridge, UK.
Abstract:
Metabolic control analysis (MCA) has provided the language and framework for quantitative study of control over flux, or over metabolites, by individual enzymes of a pathway. By contrast, top-down control analysis (TDCA) yields an immediate overview of the control structure of the whole system of interest, giving information about the control exercised by large sections of complex pathways. Unlike MCA, TDCA does not rely on the use of specific inhibitors or genetic manipulation to determine control coefficients. The method and an application of TDCA to ketogenesis are described.
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