pH-dependent pore formation in Semliki forest virus-infected Aedes albopictus cells

The plasma membrane properties of Semliki forest virus-infected Aedes cells were studied using whole-cell patch-clamp recording. Cells exhibited a marked increase in membrane conductance, gm (from 0.48 +/- 0.09 nS to 14.2 +/- 10.8 nS) upon exposure to acidic pH (5.6). The membrane potential depolarized from -40.9 +/- 5.2 mV to -2.43 +/- 7.14 mV under these conditions. In uninfected cells, there was no change in gm after lowering the pH. This implies that viral fusion proteins are involved in changing the membrane properties at low pH. The increased gm in infected cells at low pH was not persistent but declined within minutes. Millimolar concentrations of calcium and zinc prevented or reverted the increase in gm. The results suggest that viral proteins, if brought to their fusogenic conformation by exposure to low pH, form unspecific pores in the residing membrane. These pores might play a role in virus entry.