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Reovirus protein lambda 3 is a poly(C)-dependent poly(G) polymerase

M C Starnes1, W K Joklik

  • 1Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710.

Virology
|March 1, 1993
PubMed
Summary
This summary is machine-generated.

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Reovirus protein lambda 3, a viral RNA polymerase, synthesizes poly(G) from poly(C) templates. It forms stable complexes with other viral proteins but does not transcribe viral RNA.

Area of Science:

  • Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Reovirus is a non-enveloped dsRNA virus with a segmented genome.
  • Reovirus protein lambda 3 is a component of the viral core, implicated in RNA synthesis.
  • Understanding the enzymatic activity and interactions of lambda 3 is crucial for deciphering viral replication.

Purpose of the Study:

  • To isolate and characterize the enzymatic activity of reovirus protein lambda 3.
  • To investigate the substrate specificity and cofactor preference of lambda 3.
  • To examine the complex formation of lambda 3 with other reovirus proteins.

Main Methods:

  • Recombinant vaccinia virus expression system was used to produce reovirus protein lambda 3.
  • Enzymatic assays were performed using various RNA and synthetic polynucleotide templates.

Related Experiment Videos

  • Co-immunoprecipitation assays were employed to study protein-protein interactions.

    • Main Results:

    • Highly purified reovirus protein lambda 3 demonstrated transcriptase activity on poly(C) templates, producing poly(G).
    • Lambda 3 showed a preference for Mn2+ over Mg2+ as a cofactor.
    • Protein lambda 3 formed stable complexes with reovirus proteins lambda 1 and lambda 2, but these complexes lacked RNA transcriptase activity.

    Conclusions:

    • Reovirus protein lambda 3 possesses a specific RNA-dependent RNA polymerase activity, synthesizing poly(G) from poly(C).
    • Lambda 3's interaction with lambda 1 and lambda 2 is essential for forming functional viral transcription complexes.
    • Despite forming complexes, lambda 3 alone or in these complexes does not transcribe double-stranded or single-stranded reovirus RNA.