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Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134
M D Vollmer1, K Stadler-Fritzsche, M Schlömann
1Institut für Mikrobiologie, Universität Stuttgart, Federal Republic of Germany.
Archives of Microbiology
|January 1, 1993
Abstract:
2,4-Dichlorophenoxyacetate (2,4-D) in Alcaligenes eutrophus JMP134 (pJP4) is degraded via 2-chloromaleylacetate as an intermediate. The latter compound was found to be reduced by NADH in a maleylacetate reductase catalyzed reaction. Maleylacetate and chloride were formed as products of 2-chloromaleylacetate reduction, the former being funneled into the 3-oxoadipate pathway by a second reductive step. There was no indication for an involvement of a pJP4-encoded enzyme in either the reduction or the dechlorination reaction.