Related Experiment Videos
Alteration of superhelical state of DNA by aluminium (Al)
K S Rao1, B S Rao, D Vishnuvardhan
1Nutrition and Food Safety Unit, Central Food Technological Research Institute (C.F.T.R.I.), Mysore, India.
Biochimica Et Biophysica Acta
|February 20, 1993
Abstract:
The effect of aluminium (Al) on the supercoiled state of pUC18 DNA was studied by ethidium bromide fluorescence and agarose gel electrophoresis. Al at physiologically relevant concentrations relaxed the intact supercoiled DNA as well as the topoisomers induced by chloroquine. EDTA prevented the unwinding effect of Al on supercoiled DNA. Al did not alter the mobility of linear DNA in agarose gels. The implications of this finding in neurological disorders are discussed.