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Immunonephelometry and radial immunodiffusion compared for measuring serum retinol-binding protein
D J Malvy1, J D Povéda, M Debruyne
1INSERM U056, Hôpital de Bicêtre, France.
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|January 1, 1993
Summary
The Behring Nephelometer offers a rapid and accurate method for measuring retinol-binding protein (RBP) in pediatric cancer patients, comparable to traditional radial immunodiffusion assays.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Pediatric Oncology
Background:
- Accurate measurement of retinol-binding protein (RBP) is crucial for assessing nutritional status in pediatric oncology.
- Traditional methods like radial immunodiffusion (RID) can be time-consuming.
Purpose of the Study:
- To compare the accuracy and speed of a nephelometric method (Behring Nephelometer) against RID for RBP measurement.
- To evaluate RBP levels in serum samples from children with malignancies.
Main Methods:
- Serum samples from children with malignancies were analyzed using both nephelometry and RID.
- Statistical analysis included Student's t test and correlation coefficient (r) calculation.
Main Results:
- Mean RBP concentration by nephelometry was 31.0 +/- 15.6 mg/l; by RID, it was 31.2 +/- 15.7 mg/l.
- The difference between methods was not statistically significant (P = 0.6).
- A high correlation coefficient (r = 0.87) was observed between the two methods.
Conclusions:
- The Behring Nephelometer provides a rapid and accurate alternative for RBP quantification.
- Nephelometry is as reliable as RID for RBP measurement in pediatric cancer patients.