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Effect of the composition of vitrification media on survival of rabbit embryos
K Papis1, S Fujikawa, T Kojima
1National Institute of Animal Industry, Tsukuba Norindanchi, Ibaraki, Japan.
Cryobiology
|February 1, 1993
Abstract:
A total of 448 rabbit morula stage embryos were used to examine the effect of modified vitrification procedure and vitrification media containing 10% (1.36 M) glycerol, 20% (2.72 M) 1,2-propanediol, and 1 M sucrose. The best result, 89.5% embryos developed in vitro, was obtained after equilibration, vitrification, and dilution of embryos using media in which the salt concentration was maintained constant on a molar basis. Media prepared on a molal basis were more toxic and/or less efficient.