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A simple G-banding technique following nonradioactive in situ hybridization
L M Duncan1, E Dietzsch, C Rudduck
1Department of Medicine, University of Melbourne, Victoria, Australia.
Cytogenetics and Cell Genetics
|January 1, 1993
Abstract:
A simple G-banding protocol has been found to be compatible with a nonisotopic in situ hybridization technique utilizing a biotin-streptavidin-polyalkaline phosphatase complex. The chromosomes are banded following detection of the hybridization signal, the procedure requires no pretreatment of cultures or special optical equipment, and it produces permanent preparations.