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A tandemly repeated sequence from the Plasmopara halstedii genome
1Department of Crop and Weed Sciences, North Dakota State University, Fargo 58105.
Gene
|February 14, 1993
Summary
Researchers identified a tandem repeat DNA element in the Plasmopara halstedii fungus. This element can serve as a molecular probe to differentiate pathogen races, aiding in sunflower downy mildew management.
Area of Science:
- Genomics
- Mycology
- Plant Pathology
Background:
- Sunflower downy mildew, caused by Plasmopara halstedii, poses a significant threat to sunflower cultivation.
- Accurate identification of pathogen races is crucial for effective disease management strategies.
Purpose of the Study:
- To clone and characterize a repetitive DNA element from Plasmopara halstedii.
- To evaluate the potential of this element as a diagnostic tool for distinguishing pathogen races.
Main Methods:
- Cloning of a 747-bp tandem repeat element from Plasmopara halstedii.
- Sequence analysis of the cloned element.
- Identification of direct repeats and potential open reading frames (ORFs).
Main Results:
- A 747-bp tandem repeat element was successfully isolated and analyzed.
- The element contains 103 direct repeats (≥6 bp) and two potential ORFs.
- The tandem repeat element consists of four subunits, suggesting evolution via unequal crossing-over.
Conclusions:
- The cloned tandem repeat element shows potential as a molecular probe for differentiating Plasmopara halstedii races.
- This finding could lead to improved diagnostic tools for sunflower downy mildew.