Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peripherin immunoreactive structures in amyotrophic lateral sclerosis

A Migheli1, T Pezzulo, A Attanasio

  • 1Clinica Neurologica II, Università di Torino, Italy.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|February 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is there a correlation between urological and cardiovascular dysfunction in Parkinson's disease?

Functional neurology·2019
Same author

Assessment of infection in chronic wounds based on the activities of elastase, lysozyme and myeloperoxidase.

The British journal of dermatology·2015
Same author

The role of iron impurities in the toxic effects exerted by short multiwalled carbon nanotubes (MWCNT) in murine alveolar macrophages.

Journal of toxicology and environmental health. Part A·2013
Same author

Sensor for early detection of wound infection.

Biomedizinische Technik. Biomedical engineering·2013
Same author

Analysis of myeloperoxidase activity in wound fluids as a marker of infection.

Annals of clinical biochemistry·2013
Same author

Cellular and transgenic models of familial prion diseases.

Methods in molecular medicine·2011

Peripherin, a neuron-specific protein, accumulates in axonal spheroids in human spinal cords, particularly in amyotrophic lateral sclerosis (ALS). This suggests potential roles in axonal transport deficits or regeneration in neurological diseases.

Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • Peripherin levels increase in regenerating rat motor neurons.
  • Expression of peripherin in human spinal cords, both normal and diseased, was previously unknown.

Purpose of the Study:

  • To investigate peripherin expression in human spinal cords.
  • To examine peripherin in amyotrophic lateral sclerosis (ALS) and control cases.

Main Methods:

  • Immunohistochemistry and immunoelectron microscopy on human spinal cord tissue.
  • Used antibodies against peripherin, neurofilaments (NF), and ubiquitin.

Main Results:

  • Peripherin immunoreactivity observed in dorsal root ganglia, posterior columns, and roots in controls.
  • Axonal spheroids in ALS showed immunoreactivity for NF and peripherin.

Related Experiment Videos

  • Some spheroids exhibited strong peripherin and weak NF staining, distinct from ubiquitin inclusions.

    • Conclusions:

    • Peripherin and NF accumulation in spheroids may indicate impaired axonal transport.
    • High peripherin in spheroids could suggest axonal regeneration processes.