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Related Experiment Videos

Use of tetramethylbenzidine in plasma hemoglobin assay

J C Standefer, D Vanderjagt

    Clinical Chemistry
    |January 1, 1977
    PubMed
    Summary

    A new method for measuring plasma hemoglobin uses a safer chromogen, 3,3

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    Area of Science:

    • Clinical Chemistry
    • Analytical Biochemistry
    • Toxicology

    Background:

    • Benzidine is a carcinogen used in plasma hemoglobin assays.
    • A safer alternative is needed for clinical laboratory analysis.

    Purpose of the Study:

    • To evaluate 3,3',5,5'-tetramethylbenzidine (TMB) as a substitute for benzidine.
    • To assess the performance of TMB in plasma hemoglobin assays.

    Main Methods:

    • Plasma hemoglobin was assayed using TMB as the chromogenic reducing substance.
    • Assay performance was compared to benzidine-based methods.
    • Plasma inhibition was addressed through preincubation with hydrogen peroxide.

    Main Results:

    • The TMB method demonstrated comparable sensitivity, precision, and accuracy to the benzidine method.
    • The assay effectively measured plasma hemoglobin in the presence of inhibitors.

    Conclusions:

    • 3,3',5,5'-tetramethylbenzidine is a safe and effective substitute for benzidine in plasma hemoglobin assays.
    • This TMB-based assay offers a viable alternative for clinical diagnostics.

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