Atrial natriuretic factor modifies bile flow and composition in the rat

The effects of atrial natriuretic factor (ANF) on bile secretion were studied in the rat. ANF was injected 'in bolus' (5.0 micrograms/kg) every 30 min into the jugular vein. The common bile duct was cannulated and bile samples were collected every 30 min for 120 min. Systemic blood arterial pressure was registered before and after the administration of ANF. Results showed that ANF decreased bile flow and the excretion rate of sodium, potassium, chloride, bile acids, cholesterol and proteins. On the other hand, it increased pH and the excretion of bicarbonate and calcium. As ANF slightly reduces mean arterial pressure, its vascular effect may not be considered the major event. In addition, increased excretion of bicarbonate and calcium, and the fact that ANF vascular effect is short in time suggest that the peptide may have a non-vascular effect on the processes of bile formation and its modifications along the bile ducts. This extravascular effect may be exerted on the hepatocyte ions exchangers and/or at the ductal level on the processes of excretion and reabsorption of electrolytes and water.