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Determination of patulin by reversed-phase high-performance liquid chromatography with extraction by diphasic
1Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
The Analyst
|February 1, 1993
Abstract:
A simple and economical method has been developed for the determination of patulin in apple juice. The sample is extracted with ethyl acetate in a diphasic dialysis system, and the extract is cleaned up by elution from a Sep-Pak cartridge. Patulin is detected and determined by reversed-phase high-performance liquid chromatography using a Novapak C18 column and an ultraviolet detector. The lower detection limit is 1 microgram l-1 and the recovery is 85% at the 20 micrograms l-1 level.