Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Macrocyclization of polysaccharides visualized by electron microscopy

B T Stokke¹, A Elgsaeter, S Kitamura

¹Norwegian Biopolymer Laboratory, Department of Physics, University of Trondheim, NTH, Norway.

International Journal of Biological Macromolecules

|February 1, 1993

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Improved chitosan-mediated gene delivery based on easily dissociated chitosan polyplexes of highly defined chitosan oligomers.

Gene therapy·2004

Same author

Macromolecular triplex zipping observed in derivatives of fungal (1 --> 3)-beta-D-glucan by electron and atomic force microscopy.

Biopolymers·2001

Same author

The cytokine stimulating activity of (1-->3)-beta-D-glucans is dependent on the triple helix conformation.

Carbohydrate research·2000

Same author

Deconvolution can be used in electrooptic studies to correct for non-ideal electric excitation pulses only when the electric dipole moment of the studied molecules is predominantly induced.

Journal of biochemical and biophysical methods·2000

Same author

Transient electric birefringence of human erythroid spectrin dimers and tetramers at ionic strengths of 4 mM and 53 mM.

European biophysics journal : EBJ·1999

Same author

The recombinant Azotobacter vinelandii mannuronan C-5-epimerase AlgE4 epimerizes alginate by a nonrandom attack mechanism.

The Journal of biological chemistry·1999

Same journal

Investigating the effects of probiotic fermentation on the pancreatic lipase inhibitory activity of sea buckthorn polyphenol extracts by using untargeted metabolomics and multispectral analysis.

International journal of biological macromolecules·2026

Same journal

Nucleic acid and multi-omics approaches for understanding plant-microbiome interactions in grassland ecosystems.

International journal of biological macromolecules·2026

Same journal

Nanobody-based sandwich ELISA for sensitive detection of carcinoembryonic antigen-related cell adhesion molecule 5.

International journal of biological macromolecules·2026

Same journal

Digestion and fermentation characteristics of the polysaccharides from Polygalae Radix processed by Glycyrrhizae Radix et Rhizoma and its effects on gut microbiota and metabolites.

International journal of biological macromolecules·2026

Same journal

Synergistic antimicrobial and barrier enhancement in chitosan-rhamnolipid composite films for postharvest fruit preservation.

International journal of biological macromolecules·2026

Same journal

Mettl3-mediated FOXO1 mRNA translation inhibits myoblast differentiation and fusion through the MyoD1-Myomaker/Myomixer axis and the ubiquitin-proteasome system.

International journal of biological macromolecules·2026

See all related articles

Electron microscopy reveals the diverse topological structures of polysaccharides like schizophyllan, l-carrageenan, and gellan gum. These structures, including linear chains and macrocyclic formations, depend on conditions like salt concentration and polymer properties.

Area of Science:

Polymer Science
Biophysical Chemistry
Materials Science

Background:

Polysaccharides exhibit complex topological structures influencing their properties.
Understanding these structures is crucial for applications in biomaterials and drug delivery.

Purpose of the Study:

To investigate the topological features of schizophyllan, l-carrageenan, and gellan gum using electron microscopy.
To correlate observed topologies with solution conditions and polymer characteristics.

Main Methods:

Direct visualization of polysaccharide structures using transmission electron microscopy (TEM).
Analysis of structural changes induced by varying solvent conditions, salt concentrations, and thermal history.

Main Results:

Related Experiment Videos

Schizophyllan exhibits stiff, linear triple helices in native state, forming mixed topologies upon renaturation.
l-Carrageenan shows linear extended structures in salt-free solutions, transitioning to cyclic forms with salt addition.
Gellan gum forms supramolecular aggregates and macrocyclic structures in the presence of gel-promoting salts.

Conclusions:

Electron microscopy is a powerful tool for elucidating polysaccharide topology.
Polysaccharide macrocycle formation is influenced by chain stiffness, alignment, and concentration.
Environmental factors significantly dictate the topological arrangement of polysaccharides.