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Occurrence of the chromoplast protein ChrA correlates with a fruit-color gene in Capsicum annuum
M Oren-Shamir1, N Hadjeb, L A Newman
1Waksman Institute, Rutgers University, Piscataway, NJ 08855-0759.
Abstract:
Plant geneticists have determined that the color of ripe fruits of sweet peppers (Capsicum annuum L.) is determined by four genes: y, c1, c2 and cl. We have compared the electrophoretic behavior of chromoplast membrane proteins of seven varieties of C. annuum which differ in these genes. ChrA was detected only in the varieties that had a y+ genotype, and was not affected by variations in the other three genes. The identity of ChrA was verified by probing blots of SDS gels with antiserum to ChrA. The second known chromoplast-specific protein, ChrB, was found to be independent of all four genes. No proteins correlating with c1, c2 or cl were detected in either one- or two-dimensional gels.