Related Experiment Video
Updated: Aug 12, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
Cloning and characterization of a plant gene encoding a protein kinase
N Hayashida1, T Mizoguchi, K Shinozaki
1Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Ibaraki, Japan.
Abstract:
The cloning and sequence analysis of a gene that encodes a homologue of protein kinase (PK) from Arabidopsis thaliana is reported. We screened a genomic DNA library of A. thaliana using as probes oligodeoxyribonucleotides or fragments from the polymerase chain reaction that correspond to conserved regions in the catalytic domains of various PKs. One genomic clone, named Atpk5, was sequenced and analyzed. Transcripts of the corresponding gene, Atpk5, were detected in root, leaf and flower tissues by Northern blot analysis. The deduced amino acid sequence of the putative product of Atpk5 resembles those of kinases that phosphorylate ribosomal protein S6, cAMP-dependent PKs and protein kinase C. From the results of sequence comparisons, the ATPK5 protein appears to be a member of a subfamily of Ser/Thr-PKs specific to plants.
Related Concept Videos
Plant Tissue Culture
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Cell Signaling in Plants

