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Related Experiment Videos

Cloning and sequencing an ovine interleukin-4-encoding cDNA

H F Seow1, J S Rothel, P R Wood

  • 1CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.

Gene
|February 28, 1993
PubMed
Summary

Researchers successfully cloned ovine interleukin 4 (IL4) cDNA, revealing its genetic sequence and structure. This finding advances our understanding of ovine immune responses and comparative immunology.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Comparative Genomics

Background:

  • Interleukin 4 (IL4) is a critical cytokine involved in immune regulation.
  • Understanding species-specific IL4 sequences is essential for comparative immunology.
  • Ovine IL4 (ovIL4) sequence data was previously limited.

Purpose of the Study:

  • To clone and characterize the complete coding sequence of ovine interleukin 4 (IL4).
  • To facilitate comparative analysis of IL4 across different mammalian species.

Main Methods:

  • Polymerase chain reaction (PCR) was used to amplify ovine IL4 cDNA.
  • Primers were designed based on human IL4 untranslated regions.
  • RNA was isolated from stimulated ovine mesenteric lymph node cells.

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Main Results:

  • The ovine IL4 cDNA is 535 bp with a 408 nt open reading frame, encoding a 135 amino acid precursor.
  • The mature ovIL4 protein is 13.2 kDa, with two N-linked glycosylation sites and six cysteine residues.
  • Ovine IL4 shares 92% identity with bovine IL4 and lower identity with human (57%), mouse (37%), and rat (42%) IL4, due to a 51-nt deletion.

Conclusions:

  • The complete coding sequence of ovine IL4 has been successfully cloned and characterized.
  • Ovine IL4 exhibits significant sequence similarity to bovine IL4 but differs from other species due to a specific deletion.
  • This research provides valuable data for studying ovine immunity and interspecies IL4 comparisons.