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A simple modification to the LKB 7800 series Knifemaker and a balanced-break method to prepare glass knives for
1Yale University School of Medicine, Department of Cell Biology, New Haven, CT.
Journal of Microscopy
|January 1, 1993
Abstract:
A novel method of using the LKB 7800 series Knifemaker to produce glass knives using a balanced break is described. The method produces knives of sufficient quality to section aldehyde-fixed, sucrose-cryoprotected, frozen biological material in a cooled cryochamber of an ultramicrotome. The modifications to the Knifemaker are minimal and, if required, the machine can be returned easily to its normal state after use.