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Crystallization and preliminary crystallographic data for formyltetrahydrofolate synthetase from Clostridium
1Department of Chemistry and Biochemistry, University of South Carolina, Columbia 29208.
Journal of Molecular Biology
|February 20, 1993
Abstract:
Formyltetrahydrofolate synthetase from Clostridium thermoaceticum has been crystallized using the hanging-drop method from ammonium sulphate and PEG solutions. Crystals are trigonal, the space group is R32, a = 163 A, c = 259 A. A 4.2 A resolution data set has been collected. Analysis of the data using the self-rotation function shows that tetramers have approximate 222 symmetry and are positioned on a crystallographic 2-fold axis.