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Extraction of light filth from tofu: collaborative study

M J Nakashima1

  • 1U.S. Food and Drug Administration, Division of Microbiology, Washington, DC 20204.

Journal of AOAC International
|January 1, 1993
PubMed
Summary
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This study validates a new method for extracting light filth from tofu, achieving high recovery rates for both rat hairs and insect fragments. The reliable method was officially adopted by AOAC International.

Area of Science:

  • Food science
  • Analytical chemistry
  • Microbiological analysis

Background:

  • Accurate detection of light filth in food products like tofu is crucial for quality control.
  • Existing methods for light filth extraction may require optimization for specific food matrices.
  • Tofu processing can introduce potential contaminants such as insect fragments and rodent hairs.

Purpose of the Study:

  • To collaboratively study and validate a new method for the extraction of light filth from tofu.
  • To assess the efficiency and reliability of the proposed extraction technique.
  • To provide a standardized method for light filth analysis in tofu.

Main Methods:

  • A 100 g tofu sample was digested using hydrochloric acid (HCl) with Igepal CO-730 and Igepal DM-710.

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  • Light filth (rat hairs and insect fragments) was isolated via wet-sieving on a No. 230 sieve.
  • Remaining product was dispersed using Aerosol OT 75% before final filtration.

    • Main Results:

    • Collaborative study involved 9 participants analyzing spiked tofu samples.
    • Average recovery rates for rat hairs at 5, 10, and 15 fragments were 80%, 78%, and 84%, respectively.
    • Average recovery rates for insect fragments at 5, 15, and 30 fragments were 97%, 99%, and 99%, respectively.

    Conclusions:

    • The developed method demonstrates high and consistent recovery rates for light filth in tofu.
    • The method is effective and suitable for routine analysis of light filth in tofu.
    • AOAC International adopted this method as a first action official method.