Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Micro-autoradiographic method to study [18F]FDG uptake in mouse tissue

R Kubota1, S Yamada, K Kubota

  • 1Department of Radiology and Nuclear Medicine, Tohoku University, Sendai, Japan.

Nuclear Medicine and Biology
|February 1, 1993
PubMed
Summary

A novel 18F-micro-autoradiography method visualizes 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) in mouse tissues with high spatial resolution. This technique accurately quantifies tracer distribution, particularly in brain regions like the hippocampus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noninvasive Evaluation of CBF and Perfusion Delay of Moyamoya Disease Using Arterial Spin-Labeling MRI with Multiple Postlabeling Delays: Comparison with <sup>15</sup>O-Gas PET and DSC-MRI.

AJNR. American journal of neuroradiology·2017
Same author

A single-institution study examining cutaneous and non-cutaneous melanomas treated with nivolumab.

Journal of the European Academy of Dermatology and Venereology : JEADV·2016
Same author

Lymphopenia can be a useful biomarker of adverse events related to vemurafenib.

Journal of the European Academy of Dermatology and Venereology : JEADV·2016
Same author

Haemaphysalis longicornis tick bites are a possible cause of red meat allergy in Japan.

Allergy·2015
Same author

Severe rash associated with vemurafenib administration following nivolumab therapy.

Journal of the European Academy of Dermatology and Venereology : JEADV·2015
Same author

I.v. BU/fludarabine plus melphalan or TBI in unrelated cord blood transplantation for high-risk hematological diseases.

Bone marrow transplantation·2015

Area of Science:

  • Nuclear Medicine
  • Neuroscience
  • Radiochemistry

Background:

  • Accurate quantification of radiotracer distribution in tissues is crucial for understanding biological processes.
  • Existing autoradiographic methods may lack sufficient spatial resolution or require extensive exposure times.

Purpose of the Study:

  • To develop and validate a new 18F-micro-autoradiography method for detecting 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) in mouse tissues.
  • To assess the spatial resolution and optimal exposure time for the developed method.
  • To evaluate the distribution of [18F]FDG in specific regions of the mouse brain.

Main Methods:

  • Development of a micro-autoradiographic technique utilizing the positron-emitting isotope 18F.
  • Linearity assessment between silver grain counts and 18F radioactivity.

Related Experiment Videos

  • Determination of spatial resolution and optimization of exposure time (up to 7 hours) to avoid latent image fading.
  • Intravenous injection of [18F]FDG into normal mice followed by tissue analysis.

    • Main Results:

    • A linear relationship was established between silver grain number and 18F radioactivity.
    • The method achieved a spatial resolution with a half-distance of approximately 2.1 microns.
    • Optimal exposure time was determined to be 4 hours, with longer exposures causing latent image fading.
    • Highest [18F]FDG uptake in the mouse brain was observed in the hippocampal stratum lacunosum-moleculare, dentate molecular layer, and stratum oriens.
    • Neuronal cell layers showed lower tracer uptake compared to neuropil fields, with CA3a having the highest and CA4 the lowest grain levels.

    Conclusions:

    • The developed 18F-micro-autoradiography method is a sensitive and high-resolution technique for quantifying [18F]FDG distribution in mouse tissues.
    • The method requires a practical 4-hour exposure time and is applicable to other 18F-labeled tracers for assessing tracer-tissue association.
    • The findings provide valuable insights into the regional glucose metabolism in the mouse brain using [18F]FDG.