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Liposome-based flow-injection immunoassay for determining theophylline in serum
L Locascio-Brown1, A L Plant, R Chesler
1National Institute of Standards and Technology, Gaithersburg, MD 20899.
Clinical Chemistry
|March 1, 1993
Summary
We created a new flow-injection immunoassay for precise theophylline measurement in serum. This method offers a reusable and sensitive approach for quantifying theophylline levels.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Immunology
Background:
- Accurate quantification of theophylline in serum is crucial for therapeutic drug monitoring.
- Existing methods may have limitations in sensitivity, reusability, or cost-effectiveness.
Purpose of the Study:
- To develop and validate a novel heterogeneous immunoassay for the quantitative determination of theophylline in serum.
- To assess the sensitivity, reusability, and correlation with existing methods of the developed assay.
Main Methods:
- Development of a flow-injection immunoanalysis system utilizing immobilized anti-theophylline antibodies.
- Employing a competitive binding reaction between serum theophylline and theophylline-labeled liposomes.
- Utilizing liposome lysis for sensitive detection and solid-phase reactor regeneration for reusability.
Main Results:
- The developed flow-injection immunoassay provides quantitative determination of serum theophylline.
- The assay demonstrated sensitive detection capabilities.
- Excellent correlation was observed when comparing results with a commercial fluorescence polarization method.
- The immobilized antibody showed effective regeneration, allowing for reuse over hundreds of samples.
Conclusions:
- Flow-injection immunoanalysis offers a sensitive, reusable, and accurate method for serum theophylline quantification.
- This novel assay presents a viable alternative to existing theophylline measurement techniques.
- The method's reusability and sensitivity contribute to its potential for routine clinical application.