Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A theoretical evaluation of linearity

M H Kroll1, K Emancipator

  • 1Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892.

Clinical Chemistry
|March 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of archival versus newly collected tumor samples for assessing PD-L1 expression and overall survival: an updated analysis of KEYNOTE-010 trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2019
Same author

Extended pharmacologic thromboprophylaxis in oncologic liver surgery is safe and effective.

Journal of thrombosis and haemostasis : JTH·2017
Same author

Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with recurrent carcinoma of the anal canal.

Annals of oncology : official journal of the European Society for Medical Oncology·2017
Same author

Pembrolizumab as first-line therapy for patients with PD-L1-positive advanced non-small cell lung cancer: a phase 1 trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2017
Same author

PD-L1 expression and survival among melanoma patients treated with standard immunotherapy or chemotherapy.

Journal of the European Academy of Dermatology and Venereology : JEADV·2017
Same author

Endothelium-derived Relaxing Factor Inhibits Shear Stress-induced Platelet Aggregation.

Platelets·2010
Same journal

Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

Clinical chemistry·2026
Same journal

Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

Clinical chemistry·2026
Same journal

TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

Clinical chemistry·2026
Same journal

Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

Clinical chemistry·2026
Same journal

Editor's Note: Circulating Proteolytic Products of Carboxypeptidase N for Early Detection of Breast Cancer.

Clinical chemistry·2026
Same journal

In Reply to Reflexing NT-proBNP for sFlt-1/PlGF Ratios That Fall into the Measurement Uncertainty for Preeclampsia Risk Classification.

Clinical chemistry·2026
See all related articles

We propose a new quantitative method to measure linearity in analytical methods. This approach is objective and independent of assay precision, offering a more reliable evaluation than current subjective techniques.

Area of Science:

  • Analytical Chemistry
  • Method Validation
  • Biostatistics

Background:

  • Assessing method linearity is crucial for accurate analytical results.
  • Current NCCLS guidelines rely on subjective visual evaluation or imprecise lack-of-fit tests.
  • Existing methods are dependent on assay precision and lack quantitative objectivity.

Purpose of the Study:

  • To define and quantify linearity objectively.
  • To introduce a novel approach for linearity assessment independent of method precision.
  • To provide a conclusive and quantitative measure of linearity.

Main Methods:

  • Defining linearity based on the fit of a first-order polynomial compared to higher-order polynomials.
  • Utilizing the constant first derivative property of linear polynomials.

Related Experiment Videos

  • Quantifying nonlinearity by the difference between first-order and higher-order polynomial fits.

    • Main Results:

    • The proposed method provides a quantitative measure of linearity.
    • The approach is independent of the precision of the analytical method.
    • The new method offers objective and conclusive linearity assessment.

    Conclusions:

    • The proposed method offers a superior, quantitative, and objective approach to linearity assessment.
    • This method overcomes the limitations of subjective visual evaluation and precision-dependent tests.
    • It enhances the reliability and interpretability of linearity experiments in analytical method validation.