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Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
09:01

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification

Published on: September 15, 2012

Estrous-cycle-related changes in the expression of mouse endometrial and oviductal glycoproteins

H Vrcić1, B Horvat, I Damjanov

  • 1Department of Obstetrics and Gynecology, University of Zagreb, Croatia.

Gynecologic and Obstetric Investigation
|January 1, 1993
PubMed

Glycoproteins of mouse endometrium and oviduct were analyzed by lectin overlay blotting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated whole-cell extracts, sampled at each of the four phases of the normal estrous cycle. Nine endometrial and 5 oviductal glycoproteins were found only in one of these two organs or showed cyclic changes in their expression as revealed by wheat germ or Ricinus communis I lectin blotting. Glycoproteins of the same molecular weight showed different expressions in the endometrium as compared with the oviduct. Thus one can conclude that the expression of the endometrial and oviductal glycoproteins is cyclic, but organ specific.

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