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Abstract:
The unilateral kidneys of rabbits were frozen in situ for 3 min at --160 C with liquid nitrogen. After freezing, proteinuria was observed in a few rabbits. Passive hemagglutination and Ouchterlony double diffucion tests confirmed the presence of circulation antibodies against renal extracts in the serum. Immunofluorescent studies showed deposits of IgG along the renal glomerulus and in the mesangium. However, light microscopy results showed no change in renal glomerulus and electron microscopy results showed no depositions in the glomerular basement membrane. During 5 months after freezing of the kidney, glomerulonephritis has not occurred clinically and pathologically.