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High-performance liquid chromatography of human glycoprotein hormones
M A Chlenov1, E I Kandyba, L V Nagornaya
1NPO "Biotechnologia", Moscow, Russian Federation.
Journal of Chromatography
|February 12, 1993
Abstract:
The chromatographic behavior of the glycoprotein hormones from human pituitary glands and of placental origin [thyroid-stimulating hormone, luteinizing hormone and chorionic gonadotropin (CG)] was studied. It was shown that hydrophobic interaction chromatography on a microparticulate packing and anion-exchange HPLC can be applied for the purification of these hormones. Reversed-phase HPLC on wide-pore C4-bonded silica at neutral pH can be applied for the determination of the above hormones and for the isolation of pure CG and its subunits.