Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Unassigned or nonsense codons in Micrococcus luteus

A Kano¹, T Ohama, R Abe

¹Department of Biology School of Science, Nagoya University, Japan.

Journal of Molecular Biology

|March 5, 1993

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genomic correlates of response and resistance to the irreversible FGFR1-4 inhibitor futibatinib based on biopsy and circulating tumor DNA profiling.

Annals of oncology : official journal of the European Society for Medical Oncology·2024

Same author

Community pharmacy-level factors associated with medical and nursing home facility collaboration in Japan.

Die Pharmazie·2019

Same author

Finasteride 1 mg has no inhibitory effect on omeprazole metabolism in extensive and poor metabolizers for CYP2C19 in Japanese.

European journal of clinical pharmacology·2006

Same author

pH-sensing nano-crystals of carbonate apatite: effects on intracellular delivery and release of DNA for efficient expression into mammalian cells.

Gene·2006

Same author

Immunolocalization of centriole-associated striated rootlets in human submandibular gland cells with and without solitary cilia.

The Histochemical journal·2002

Same author

Localization of gamma-tubulin to the basal foot associated with the basal body extending a cilium.

The Histochemical journal·2001

Same journal

DynaPIN: A tool for characterizing dynamic protein interfaces.

Journal of molecular biology·2026

Same journal

BindRNAgen: Protein-binding RNA sequence generation using latent diffusion models.

Journal of molecular biology·2026

Same journal

Structural basis of HSP90C, a highly active chloroplastic HSP90 chaperone from Arabidopsis thaliana.

Journal of molecular biology·2026

Same journal

Clinical inflammasome biomarkers: Progress and prospects.

Journal of molecular biology·2026

Same journal

Biologically Relevant, Cationic Residues in Human Rhinovirus Stabilize Capsid-Bound RNA Duplexes, and Restrict Capsid Flexibility.

Journal of molecular biology·2026

Same journal

Cryo-EM structures of phage T4 infection intermediate.

Journal of molecular biology·2026

See all related articles

In Micrococcus luteus, AGA and AUA codons do not code for amino acids or stop signals. These unassigned codons halt translation without releasing the peptide, unlike UGA stop codons.

Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Certain codons ending in A are absent in Micrococcus luteus.
This suggests potential unassigned codons in this bacterium.

Purpose of the Study:

To investigate if AGA and AUA codons are unassigned in M. luteus.
To determine the translational behavior of these codons.

Main Methods:

Utilized cell-free extracts from M. luteus.
Translated synthetic messenger RNAs with specific codons (AGA, AUA, UGA).
Analyzed peptide synthesis and ribosome association via sucrose-gradient centrifugation.

Main Results:

Translation ceased at AGA and AUA codons, with peptides remaining attached to ribosomes.

Related Experiment Videos

UGA codons also halted translation but resulted in peptide release from ribosomes.

AGA and AUA function differently from UGA termination codons.

Conclusions:

AGA and AUA are likely unassigned codons in M. luteus.
These codons function as translational stops but not as release signals.
This finding expands our understanding of codon reassignment and genetic code variations.