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Updated: May 25, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
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Comparison of two methods for computing exogenous substrate oxidation using 13C-labeling

F Péronnet1, E Adopo, D Massicotte

  • 1Département D'Education Physique, Université de Montréal, Québec, Canada.

Medicine and Science in Sports and Exercise
|February 1, 1993
PubMed
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This study measured exogenous glucose oxidation rates during exercise using 13C-glucose tracer and two calculation methods. Results showed consistent oxidation rates across different enrichment levels, aiding exercise metabolism research.

Area of Science:

  • Exercise Physiology
  • Nutritional Biochemistry
  • Stable Isotope Tracing

Background:

  • Accurate measurement of exogenous glucose oxidation is crucial for understanding exercise metabolism.
  • Previous methods using 14C-glucose have limitations.
  • Stable isotopes like 13C offer a safer alternative for tracing substrate utilization.

Purpose of the Study:

  • To measure exogenous glucose oxidation rates during exercise using 13C-glucose.
  • To evaluate two computation procedures accounting for endogenous substrate oxidation (Rendo).
  • To compare 13C-glucose oxidation results with existing 14C-glucose studies.

Main Methods:

  • Eight subjects performed four exercise trials (68% VO2max, 90 min) on a cycle ergometer.
  • 30g of 13C-glucose at varying enrichment levels was ingested post-30 min exercise.

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  • CO2 analysis measured 13C enrichment to calculate glucose oxidation, accounting for Rendo.

    • Main Results:

    • Rendo significantly increased from rest to exercise.
    • Exogenous glucose oxidized in the last hour of exercise was 14.9 ± 4.4 g (Trials A/B) and 13.0 ± 4.2 g (Trial C).
    • These amounts represented 7.4% and 6.3% of total energy expenditure, respectively.

    Conclusions:

    • Two computation procedures using 13C-glucose provided comparable exogenous glucose oxidation rates during exercise.
    • The method effectively accounts for endogenous substrate contributions to CO2.
    • 13C-glucose is a viable tracer for quantifying substrate utilization during physical activity.