Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interleukin-1 binding sites on astrocytes

E M Ban1, L L Sarliève, F G Haour

  • 1Unité de Pharmacologie Neuro-Immuno-Endocrinienne, Institut Pasteur, Paris, France.

Neuroscience
|February 1, 1993
PubMed
Summary

Interleukin-1 (IL-1) binds to receptors on mouse astroglial cells, but not microglia. This binding supports IL-1's role in central nervous system responses, like scar tissue formation after brain injury.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changes in brain components during the development of mice homozygous for the locus "dwarf" (dw)(1,5.).

Neurochemical research·2013
Same author

Investigation of myelination in vitro: polar lipid content and fatty acid composition of myelinating oligodendrocytes in rat oligodendrocyte cultures.

Neurochemistry international·2010
Same author

Ontogeny of steroid metabolizing enzymes in rat oligodendrocytes.

The Journal of steroid biochemistry and molecular biology·2001
Same author

Adrenal gland SNAP-25 expression is altered in thyroid hormone receptor knock-out mice.

Neuroreport·2001
Same author

Phospholipid composition in late infantile neuronal ceroid lipofuscinosis.

European journal of clinical investigation·2000
Same author

Rat oligodendrocytes express the vitamin D(3) receptor and respond to 1,25-dihydroxyvitamin D(3).

Glia·2000

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Interleukin-1 (IL-1) influences glial cell functions in the central nervous system.
  • Understanding IL-1 receptor distribution is crucial for elucidating its role in neurological processes.

Purpose of the Study:

  • To investigate the specific binding capacity of astroglial cells for recombinant iodinated human interleukin-1 alpha (IL-1α).
  • To determine the presence and characteristics of IL-1 receptors on astrocytes and microglia in the mouse brain.

Main Methods:

  • In situ and in vitro autoradiography on mouse brain sections and primary glial cell cultures (astrocytes and microglia).
  • Quantitative autoradiography and competition binding experiments using iodinated human IL-1α, IL-1 receptor antagonist, and mouse IL-1β.

Main Results:

  • Specific binding of iodinated human IL-1α was observed in areas of glial proliferation in brain sections.
  • Quantitative analysis revealed EC50 values of 5 x 10⁻¹¹ M for human IL-1α and ~10⁻⁹ M for the IL-1 receptor antagonist.
  • In cultures, IL-1α specifically bound to astrocytes but not microglia, with EC50 values of 8 x 10⁻¹¹ M for IL-1α and 1 x 10⁻¹⁰ M for mouse IL-1β.

Conclusions:

  • Astroglial cells possess specific binding sites for IL-1.
  • These findings provide biochemical evidence for IL-1 receptors on astrocytes, supporting their role in mediating IL-1 effects in the CNS.
  • This may involve astroglial proliferation contributing to scar tissue formation post-brain injury.

Related Experiment Videos