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Amplification of Mycoplasma iowae using polymerase chain reaction
1Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
Avian Diseases
|January 1, 1993
Abstract:
Based on sequence data of Mycoplasma iowae recombinant DNA probe, pMI-12, two 25-base primers were synthesized for use in the polymerase chain reaction (PCR). An M. iowae species-specific 299-base pair product was amplified by the primers. An annealing temperature of 58.5 C was critical for detecting all members of this heterogeneous species while maintaining specificity of the M. iowae PCR. The minimum amount of target DNA detected by M. iowae PCR was 1 pg, which was 1000 times more sensitive than the dot-blot assay using M. iowae recombinant DNA probes.