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Related Experiment Videos

The mouse relaxin gene: nucleotide sequence and expression

B A Evans1, M John, K J Fowler

  • 1Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.

Journal of Molecular Endocrinology
|February 1, 1993
PubMed
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Researchers identified the mouse relaxin gene and its ovarian expression during pregnancy. They discovered a unique tyrosine insertion in the A chain of mouse relaxin, potentially affecting its function.

Area of Science:

  • Molecular Endocrinology
  • Reproductive Biology
  • Genomics

Background:

  • Relaxin is a crucial polypeptide hormone regulating collagen remodeling and uterine contractility, particularly during pregnancy.
  • Understanding relaxin's molecular characteristics is vital for comprehending reproductive physiology.

Purpose of the Study:

  • To establish the sequences of relaxin cDNAs from pregnant mouse ovaries.
  • To investigate the structure and genomic organization of the mouse relaxin gene.
  • To determine the expression pattern of relaxin during mouse pregnancy.

Main Methods:

  • Sequencing of relaxin complementary DNAs (cDNAs) derived from ovaries of late-pregnant mice.
  • Polymerase chain reaction (PCR) amplification and subclone analysis.

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  • Direct sequencing of relaxin peptides.
  • Analysis of the mouse genome for relaxin gene copy number.
  • Detection of relaxin gene expression in various reproductive tissues.

    • Main Results:

    • Identified mouse relaxin cDNAs encoding proteins identical except for isoleucine or valine at position 11 in the A chain.
    • Discovered a consistent insertion of an extra tyrosine residue before the final A chain cysteine in all ovarian relaxin cDNAs.
    • Confirmed the presence of a single relaxin gene in the mouse genome.
    • Demonstrated significant relaxin gene expression in the ovary during pregnancy, with undetectable levels in the placenta, uterus, and fetus.

    Conclusions:

    • The mouse relaxin gene is expressed in the ovary during pregnancy, with a unique structural feature (tyrosine insertion) in the A chain.
    • This tyrosine insertion may influence relaxin's folding and interaction with its receptor, warranting further structure-activity studies.
    • Ovarian expression is the primary source of relaxin during mouse pregnancy, not the placenta, uterus, or fetus.